Imaging neuropeptide release at synapses with a genetically engineered reporter

eLife

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[1] Department of Neurobiology, University of Southern California, Los Angeles, United States;Department of Neurobiology, University of Southern California, Los Angeles, United States;Neuroscience Graduate Program, University of Southern California, Los Angeles, United States;Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States;Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States;Howard Hughes Medical Institute, California Institute of Technology, Pasadena, United States;Tianqiao and Chrissy Chen Institute for Neuroscience, California Institute of Technology, Pasadena, United States;Oak Crest Institute of Science, Monrovia, United States;

关键词: neuropeptides;    dense core vesicle;    neurotransmission;    D. melanogaster;   

DOI  :  10.7554/eLife.46421

来源: publisher

【 摘 要 】

10.7554/eLife.46421.001Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.

【 授权许可】

CC BY