Size control of the inner ear via hydraulic feedback

eLife

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[1] Department of Systems Biology, Harvard Medical School, Boston, United States;School of Engineering and Applied Sciences, Harvard University, Cambridge, United States;School of Engineering and Applied Sciences, Harvard University, Cambridge, United States;Department of Organismal and Evolutionary Biology, Harvard University, Cambridge, United States;Department of Physics, Harvard University, Cambridge, United States;Kavli Institute for NanoBio Science and Technology, Harvard University, Cambridge, United States;

关键词: growth;    size control;    pressure;    inner ear;    transepithelial flow;    systems modeling;    Zebrafish;   

DOI  :  10.7554/eLife.39596

来源: publisher

【 摘 要 】

10.7554/eLife.39596.001Animals make organs of precise size, shape, and symmetry but how developing embryos do this is largely unknown. Here, we combine quantitative imaging, physical theory, and physiological measurement of hydrostatic pressure and fluid transport in zebrafish to study size control of the developing inner ear. We find that fluid accumulation creates hydrostatic pressure in the lumen leading to stress in the epithelium and expansion of the otic vesicle. Pressure, in turn, inhibits fluid transport into the lumen. This negative feedback loop between pressure and transport allows the otic vesicle to change growth rate to control natural or experimentally-induced size variation. Spatiotemporal patterning of contractility modulates pressure-driven strain for regional tissue thinning. Our work connects molecular-driven mechanisms, such as osmotic pressure driven strain and actomyosin tension, to the regulation of tissue morphogenesis via hydraulic feedback to ensure robust control of organ size.Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).

【 授权许可】

CC BY