Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond

eLife

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[1] Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States;

关键词: macrophage;    genome editing;    host-pathogen interactions;    tuberculosis;    Listeria monocytogenes;    Mouse;   

DOI  :  10.7554/eLife.45957

来源: publisher

【 摘 要 】

10.7554/eLife.45957.001Macrophages play critical roles in immunity, development, tissue repair, and cancer, but studies of their function have been hampered by poorly-differentiated tumor cell lines and genetically-intractable primary cells. Here we report a facile system for genome editing in non-transformed macrophages by differentiating ER-Hoxb8 myeloid progenitors from Cas9-expressing transgenic mice. These conditionally immortalized macrophages (CIMs) retain characteristics of primary macrophages derived from the bone marrow yet allow for easy genetic manipulation and a virtually unlimited supply of cells. We demonstrate the utility of this system for dissection of host genetics during intracellular bacterial infection using two important human pathogens: Listeria monocytogenes and Mycobacterium tuberculosis.

【 授权许可】

CC BY