A Comparison between CAPS and SCAR Markers in the Detection of Resistance Genes in some Tomato Genotypes against Tomato Yellow Leaf Curl Virus anfiles\vol12\n2\Paper number 1.pdfd Whitefly

Jordan Journal of Biological Sciences

Sherin A. Mahfouze¹ 

关键词: Solanum lycopersicon;    Wild tomato;    Ty-1;    Ty-2;    Ty-3;    Mi-1.2;    Disease resistance.;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

The Tomato yellow leaf curl virus (TYLCV) is transmitted by the whitefly vector (Bemisia tabaci) and can cause big losses in tomato yields in Egypt and worldwide. Several genes linked to resistance to TYLCV (Ty-1, Ty-2 and Ty-3) and whitefly (Mi-1.2) in wild species and cultivated tomatoes were detected. Cleaved amplified Polymorphic Sequence (CAPS) and Sequence Characterized Amplified Region (SCAR) markers are used as tools in tomato lines carrying different combinations of Ty-1, Ty-2, Ty-3 and Mi-1.2 alleles. In this study, a total of nineteen tomato genotypes have been selected for the existence of resistance alleles, using five CAPS and three SCAR markers. Resistant allele-specific fragments for TYLCV and whitefly have been identified in some of the genotypes used in the current research. Thus, CAPS markers closely linked to Ty-1 and Mi-1.2 discriminated homozygous and heterozygous tomato genotypes. In addition, Sequence Characterized Amplified Region (SCAR) markers linked to Ty-2 and Ty-3 genes separated both dominant and recessive alleles in tomato plant materials. On the contrary, CAPS (Ty-2 and Ty-3) and SCAR (Mi-1.2) markers gave false positive results. These molecular markers are considered advantageous tools for pyramiding resistance genes of several genotypes into a single line, improving the resistance to begomoviruses.

【 授权许可】

CC BY