PREPROCESSING AND REGISTRATION OF MINISCOPE-BASED CALCIUM IMAGING OF THE RODENT BRAIN

International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences

Brazhe, A. R.^2¹  Sagindykov, T. B.^1² 

[1] Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia^2;Laboratory of Mathematical Methods of Image Processing, Faculty of Computational Mathematics and Cybernetics, Lomonosov Moscow State University, Moscow, Russia^1

关键词: image registration;    neurons;    live tissue imaging;    miniscope;   

DOI  :  10.5194/isprs-archives-XLII-2-W12-185-2019

学科分类：地球科学(综合)

来源: Copernicus Publications

【 摘 要 】

Microscopic imaging is central to the brain and cognition studies in animals and often requires advanced image processing. In vivo recordings on awake behaving animals require stabilization of the images as the tissue in the images undergoes non-rigid deformations due to animal movement, pulse beat and breathing of the animal. Here we propose an approach to compensation for the tissue motion in calcium imaging data acquired with miniaturized wearable microscopes (miniscopes) from live rodent brains. Our approach includes preprocessing of the images in which we compensate for non-uniform illumination, remove calcium transients and instrument-specific noise. For image registration we use the multiscale mutual information based non-rigid algorithm with B-spline transformation model. We present the preliminary results of such motion compensation approach applied to the real miniscope image stacks.

【 授权许可】

CC BY