Evaluation of Six Imported Accessions of Lupinus albus for Nutritional and Molecular Characterizations under Egyptian Conditions

Jordan Journal of Biological Sciences

Sherin A. Mahfouze¹ 

关键词: White lupin;    yield;    isozyme;    RAPD;    ISSR.;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

White lupin (Lupinus albus L.) is an annual grain-legume widely harvest and cultivated in Egypt and also worldwide. Lupin seeds are utilized as food for the human and livestock nutrition. The aim of the present study is to estimate the genetic diversity among the seven genotypes of L. albus. In addition, the field performance of these genotypes was evaluated to drought tolerant under Egyptian conditions. Also, the macro- and microelements in the seeds of the white lupin were determined. In the present investigation, high significant differences among seven white lupin genotypes were observed for field performance traits under water deficit. Two accessions L. albus CGN 10106 and CGN 10108 were tolerant of drought compared with other genotypes. Thus, these genotypes recorded the highest seed yield when exposed to water0T 0Tstress. The macro- and microelement contents of seven white lupin genotypes were found to be different based on the genotype. In the present study, slight differences were observed in the total protein bands among the seven genotypes, thus polymorphism is low (28.57% polymorphism). These genotypes varied in expression from strong to low both Polyphenol Oxidase (PPO) and Peroxidase (POX) isozymes. So, the highest expression of antioxidant enzymes was recorded in genotypes tolerant of drought CGN 10106 and CGN 10108. On the other hand, Random Amplified Polymorphism DNAs (RAPD); Inter Simple Sequence Repeats (ISSR) assays recorded the percentage of polymorphism 47.96% and 29.82%, respectively. The Nei genetic similarity index ranged from 0.74 to 0.88 using UPGMA. These results are important in the breeding programs for the selection process of parental strains that feasibility the prediction of crosses to generate hybrids with the best performance.

【 授权许可】

CC BY