Molecular Detection of Putative Virulence Factors (Fungalysin and Subtilisin) in Aspergillus tamarii Isolated from Human Skin

Jordan Journal of Biological Sciences

Oluwole M. David¹ 

关键词: Aspergillosis;    Aspergillus tamari;    Cutaneous infections;    Fungalysin;    Subtilisin;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

The genus Aspergillus is widely distributed containing a number of species that are opportunistic pathogens for both humans and animals. Some of the species of this genus were identified based on morphology. However, the molecular detection of the pathogens is necessary. The detection of the virulence factors of Aspergillus tamarii associated with the human skin was performed in this study. Aspergillus tamarii isolated from skin infections were examined using morphological and molecular criteria. The phylogenetic relationship of isolates was determined using sequences from the ITS gene. Fungalysin (MEP3) and subtilisin (SUB1) genes were also detected in the isolates using Polymerase Chain Reaction. Out of ten morphologically identified Aspergillus tamarii seven were identified to be Aspergillus tamarii using molecular methods. All the molecularly identified A. tamarii had fungalysin (MEP3) gene while the subtilisin (SUB1) gene was detected in six out of seven isolates. The nucleic acid sequencing offers an additional tool for a better diagnoses and understanding of the phylogenetic relationships within genus or species of pathogenic microbes.

【 授权许可】

CC BY