Quantitative Disease Resistance Loci towards Phytophthora sojae and Three Species of Pythium in Six Soybean Nested Association Mapping Populations

Crop Science

Veney, Deloris^1¹  Balk, Christine^2²  Scott, Kelsey^1³  McHale, Leah K.^3⁴ 

[1] Dep. of Horticulture and Crop Science, The Ohio State Univ., 2021 Coffey Rd., Columbus, OH 43210 Center for Applied Plant Sciences and Center for Soybean Research, The Ohio State Univ., Columbus, OH 43210^3;Dep. of Plant Pathology, The Ohio State Univ., Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691, current address, The Davey Tree Expert Company, 1500 N Mantura St., Kent, OH 44240^2;Dep. of Plant Pathology, The Ohio State Univ., Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691^1;KDep. of Plant Pathology, The Ohio State Univ., Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691 Center for Applied Plant Sciences and Center for Soybean Research, The Ohio State Univ., Columbus, OH 43210^4

关键词: BLUP;    best linear unbiased predictor;    LOD;    logarithm of the odds;    NAM;    nested association mapping;    QDRL;    quantitative disease resistance locus/loci;    QTL;    quantitative trait locus/loci;    RCBD;    randomized complete block design;    RIBD;    randomized incomplete block design;    RIL;    recombinant inbred line;    SoyNAM;    Soybean Nested Association Mapping;   

DOI  :  10.2135/cropsci2018.09.0573

学科分类：农业科学（综合）

来源: Crop Science

【 摘 要 】

Comparison of quantitative disease resistance loci (QDRL) towards the diverse array of soilborne pathogens that affect soybean [Glycine max (L.) Merr.] is key to the incorporation of resistance in breeding programs. The water molds Phytophthora sojae (Kauffman & Gerdmann), Pythium irregulare (Buisman), Pythium ultimum var. ultimum (Trow), and Pythium ultimum var. sporangiiferum (Drechsler) contribute to soybean yield losses annually. Six Soybean Nested Association Mapping (SoyNAM) populations were evaluated for resistance to one or more of these pathogens. Four were screened with a tray test to measure lesion length after inoculation with Ph. sojae; cup assays were used to screen three, three, and two populations for resistance towards Py. irregulare, Py. ultimum var. ultimum, and Py. ultimum var. sporangiiferum, respectively. There were two to eight major or minor QDRL identified within each SoyNAM population towards one or more of these water molds for a total of 33 QDRL. The SoyNAM populations evaluated for resistance to two or more water molds had different QDRL towards each pathogen, indicating that within a source of resistance, mechanisms are potentially specific to the pathogen. Only 3 of the 33 QDRL were associated with resistance to more than one pathogen. There was a major QDRL on chromosome 3 associated with resistance to Py. ultimum var. ultimum and Py. ultimum var. sporangiiferum, and QDRL on chromosomes 13 and 17 shared a flanking marker for both Py. irregulare and Py. ultimum var. ultimum. The SoyNAM population can serve as a diverse resource to map QDRL and compare mechanisms across pathogens and isolates.

【 授权许可】

CC BY