Identification of Quantitative Trait Loci for Grain Yield and Other Traits in Tropical Maize Under High and Low Soil-Nitrogen Environments

Crop Science

Ribeiro, P.F.^1¹  Danquah, E.Y.^3²  Badu-Apraku, B.^2³  Gracen, V.E.^3⁴ 

[1] CSIR– Crops Research Institute (CRI), P. O. Box 3785, Fumesua, Kumasi, Ghana West Africa Centre for Crop Improvement, Univ. of Ghana, Legon, Ghana^1;CSIR– Crops Research Institute (CRI), P. O. Box 3785, Fumesua, Kumasi, Ghana^4;International Institute of Tropical Agriculture, P.M.B. 5320, Ibadan, Nigeria^2;West Africa Centre for Crop Improvement, Univ. of Ghana, Legon, Ghana^3

关键词: ASI;    anthesis–silking interval;    CIM;    composite interval mapping;    CSIR;    Council for Scientific and Industrial Research;    DTA;    days to anthesis;    DTS;    days to silk;    EHT;    ear height;    EPP;    ears per plant;    GEI;    genotype by environment interaction;    GY;    grain yield;    HN;    High Nitrogen;    h 2;    Broad sense heritability;    IITA;    International Institute of Tropical Agriculture;    LOD;    logarithm of the odds;    MAS;    Marker assisted selection;    P;    plant aspect;    PHT;    Plant height;    PVE;    phenotypic variance explained;    QTL;    Quantitative trait locus;    QTLs;    Quantitative trait loci;    SG;    stay-green;    SGC;    stay-green characteristic;   

DOI  :  10.2135/cropsci2017.02.0117

学科分类：农业科学（综合）

来源: Crop Science

【 摘 要 】

Low soil Nitrogen (low-N) is one of the most important abiotic stressors responsible for significant yield losses in maize (Zea mays L.). The development and commercialization of low-N–tolerant genotypes can contribute to improved food security in developing countries. However, selection for low-N tolerance is difficult because it is a complex trait with strong interaction between genotypes and environments. Marker-assisted breeding holds great promise for improving such complex traits more efficiently and in less time, but requires markers associated with the trait of interest. In this study, 150 BC₂F₁ families of CML 444 × CML 494 were evaluated at two locations for two consecutive seasons to identify SNP markers associated with quantitative trait loci (QTL) for yield and other agronomic traits under low- and high-N environments. A total of 13 QTL were identified with 158 SNP markers, of which nine and four QTL were detected under low- and high-N environments, respectively. Five QTL one each for grain yield (qgy-1), days to silking (qdts-1) and anthesis- silking interval (qasi-6), and two for stay green characteristic (qsg-1 and qsg-4) were close to their adjacent markers, with an interval of 0.7 to 5.2 cM between them and explained phenotypic variance of 9 to 21%. These QTL would be invaluable for rapid introgression of genomic regions into maize populations using marker-assisted selection (MAS) approaches. However, further validation of these QTL is needed before use in MAS.

【 授权许可】

CC BY