Isolation of Blood Group Non-specific Lectin from Calotropis gigantean Seeds

Jordan Journal of Biological Sciences

Vishwanath B. Chachadi¹ 

关键词: Lectins;    Glycans;    Hemagglutination;    Calotropis gigantean;    Hapten;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

An altered expression of glycans on the cell surface can act as a marker of various diseases including cancer and AIDS. The identification of these altered glycans can be easily achieved by using glycan binding proteins, specifically antibodies and lectins. Identifying lectins, specific towards carbohydrates that are markers of various diseases will help towards an early diagnosis of diseases. The present study describes the isolation, carbohydrate specificity, and heat stability of lectin from the Calotropis gigantea seeds. Calotropis gigantea lectin (CGL), showed a blood group non-specificity strongly inhibited by glycans of mucin glycoprotein. The Ammonium sulphate precipitation of Calotropis gigantean crude extract results in the concentration of hemagglutination activity at 30-60 % saturation. Lectin retained its activity when exposed to as high as 50°C for one hour. Since Calotropis gigantean is commonly used as a medicinal plant, lectin from this plant may have haematological applications and can be used in the purification of glycoproteins.

【 授权许可】

CC BY