Mycosynthesis of Silver Nanoparticles using Terminia sp. Desert Truffle, Pezizaceae, and their Antibacterial Activity

Jordan Journal of Biological Sciences

Mustafa N. Owaid¹ 

关键词: Ascocarp;    Tuber;    Antibacterial effects;    Green nanotechnology;    Fungi.;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Desert truffle Tirmania sp. is appropriate as a greener reducing agent to synthesize silver nanoparticles at room temperature in a dark place by utilizing the aqueous extract of ascocarps of Tirmania sp. and 10P-3PAgNOR3R solution. Characterization of those nanoparticles was accomplished by changing color, UV-visible spectrum, FT-IR, AFM and SEM analyses. The changing in color is considered a first indicator for the formation of AgNPs which changed from pale yellow to dark brown after seventy-two hours. UV-visible spectrum showed the formation of AgNPs at peak 415 nm. AFM and SEM images demonstrated nanoparticles with spherical or irregular-shaped, agglomerated and highly heterogeneous. The higher zone of inhibition was 14 mm against Pseudomonas aeruginosa at a concentration of 5 mg/well while, the lower zone of inhibition was 9.5 mm toward Staphylococcus aureus at the level of 5 mg/well. These Tirmania-AgNPs had a promising antibacterial activity toward gram-negative and gram-positive bacteria, mainly the infectious bacteria of the eyes, Pseudomonas aeruginosa.

【 授权许可】

CC BY