Determination of the Immunogenic and Hematologic Effects of Titanium Nanoparticles Manufactured fromAspergillus flavus in Vivo

Jordan Journal of Biological Sciences

Mohammed N. Maaroof¹ 

关键词: TiO2 NPs;    Hematological parameters;    Immunoglobulin level;    Cytokines;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

The Titanium dioxide nanoparticles (TiO2 NPs) have been synthesized via biological methods using Aspergillus flavus, and were subjected to numerous tests to confirm the formation of nanoparticles such as UV visible spectroscopy X-ray diffraction (XRD), Fourier transform infrared (FTIR), Atomic force microscope (AFM) and Scanning electron microscope (SEM) analysis. This study evaluates the immunogenic and hematologic effects of TiO2 NPs in vivo in rabbit groups as treatment after infection by two pathogenic bacteria; Escherichia coli (G1) and Staphylococcus aureus (G2). A dosage of 20 mg/kg of TiO2 NPs was administrated orally to the rabbit groups (G1, G2) and the results were compared with the control group (G3) which received the same dosage of TiO2 NPs without any prior bacterial infection. The infected groups showed a significant reduction in the values of RBC, PCV, Hb compared with the control group (G3) and the groups (TG1, TG2, TG3) that were treated with TiO2 NPs. Neutrophils percentages were higher than Lymphocytes in the infected groups compared with the control group before and after the treatment. The titers of IgG and IgM showed a significant increase in the infected animals compared with the control group and the group that was administrated TiO2 NPs only. The levels of IL-10, IL-6, IFN-γ and TNF-α showed a significant elevation (P 0.05) in group (TG3) compared with the control group (G3).

【 授权许可】

CC BY