Prevalence and Phylogenetic Analyses of Trichuris suis in Pigs in Hunan Province, Subtropical China

The Korean Journal of Parasitology

Lei Tan^1^2^3¹  Yisong Liu^1²  Aibing Wang^1^2^3³  Jing Yi^1⁴ 

[1] College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, P.R., China^1;College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, P.R., China^4;Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), Changsha, Hunan, 410128, P.R., China^2;R & D Center for Animal Reverse Vaccinology of Hunan Province, South China Agricultural University, Guangzhou, Guangdong Province 510642, P.R., China^3

关键词: Trichuris suis;    prevalence;    pig;    internal transcribed spacer (ITS);    Hunan province;   

DOI  :  10.3347/kjp.2018.56.5.495

学科分类：生物科学（综合）

来源: The Korean Society for Parasitology

【 摘 要 】

Trichuris suis infection in pigs is ubiquitous in intensive and extensive farms, which causes potential threat to human health. The objective of this research was to investigate the prevalence of T. suis in pigs in Hunan province. Total 2,267 fresh fecal samples distributed in 28 pig farms from 7 different administrative regions (Hunan province) were evaluated for the existence of T. suis eggs using saturated NaCl floating method. The average infection rate of T. suis in pigs was 8.91% in Hunan province. To determine genetic variation of the gained T. suis isolates in the present study, the internal transcribed spacer (ITS) regions from nuclear ribosomal DNA (rDNA) of 7 T. suis isolates were cloned and analyzed. Nucleotide diversities were 1.0–3.5% and 0–3.8% for ITS-1 and ITS-2, respectively. Phylogenetic analyses indicated that all isolates collected in the present study and T. suis available in Genbank generated a monophyletic clade. The present investigation revealed high infection rates of T. suis in pigs in Hunan province, which shed light on making effective measures to prevent and control T. suis infection in pigs in Hunan province.

【 授权许可】

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