Lability in sulfur acidic cultivation medium explains unstable effects of CDK inhibitors on Cyanidioschyzon merolae cell proliferation

The Journal of General and Applied Microbiology

Yuki Kobayashi¹  Kan Tanaka² 

[1] Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology;Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology,Core Research for Evolutional Science and Technology, Japan Science and Technology Agency

关键词: CDK;    cell cycle;    Cyanidioschyzon merolae;    inhibitor;    red algae;   

DOI  :  10.2323/jgam.2018.01.002

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

We previously showed that nuclear DNA replication (NDR) is regulated by a checkpoint monitoring the occurrence of organelle DNA replication (ODR) in a unicellular red alga Cyanidioschyzon merolae. These analyses depended on the use of chemical CDK inhibitors such as CDK2 inhibitor II and roscovitine, but subsequent analyses yielded conflicting results depending on the experimental conditions. In the present study, we identified significantly short half-lives of the used chemicals in the sulfur acidic cultivation medium, which reconciles the discrepancy among these results.

【 授权许可】

Unknown