Acta Chromatographicae | |
A UHPLC–MS/MS method for the quantitation of olmutinib in rat plasma | |
Bao Su-su1  | |
[1] School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang | |
关键词: Olmutinib; UHPLC–MS/MS; pharmacokinetics; non-small cell lung cancer; | |
DOI : 10.1556/1326.2018.00375 | |
学科分类:化学(综合) | |
来源: Akademiai Kiado Rt. | |
【 摘 要 】
Olmutinib (Olita™) is an oral third-generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) which is used to treat non-small cell lung cancer (NSCLC). A simple, rapid, and sensitive method based on ultra-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) has been developed for the determination of olmutinib. Sample preparation was performed following simple one-step protein precipitation with acetonitrile. Olmutinib and internal standard (dasatinib) were separated on an Eclipse Plus C18 RRHD (2.1 × 50 mm, 1.8 μm) column. The mobile phase consisted of acetonitrile–0.1% formic acid in water with gradient elution. A total run time of 1.7 min was achieved. Detection was performed on a positive-ion electrospray ionization mass spectrometer in multiple reaction monitoring (MRM) mode, using transitions of m/z 487.2 → 402.1 for olmutinib and m/z 488.2 → 401 for dasatinib (IS), respectively. The calibration curve (R2 = 0.999) was linear over the range of 1–500 ng/mL. The recovery of olmutinib ranged from 85.8% to 95.5%. This method can be applied to pharmacokinetic studies of olmutinib.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201910285054297ZK.pdf | 454KB | download |