Simultaneous quantitative determination of active components in Tetrastigma hemsleyanum by RP-HPLC coupled with diode array detection

Acta Chromatographicae

Zhu Yanqin¹  Du Ping²  Yin Qinhong³ 

[1] Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, Yunnan;Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan;Research Center for Analysis and Measurement, Kunming University of Science and Technology, Kunming 650093

关键词: Active components;    Tetrastigma hemsleyanum;    RP-HPLC;    diode array detection;   

DOI  :  10.1556/1326.2017.00231

学科分类：化学（综合）

来源: Akademiai Kiado Rt.

【 摘 要 】

An effective, reliable, and sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) with diode array detector (DAD) method was investigated for simultaneous determination of polydatin, isoquercitrin, resveratrol, and nicotiflorin in Tetrastigma hemsleyanum. The chromatographic separation of the four compounds was carried out on a Welchrom ODS column (4.6 mm × 250 mm, 5 μm) by gradient elution with phosphoric acid (H3PO4) aqueous solution (0.4%)–methanol as the mobile phase, at the temperature of 30 °C and a flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm. Under optimum conditions, the baseline separation of these four compounds can be performed within 30 min. The developed method was validated in terms of detection limit, quantification limit, linearity, precision, and recovery tests. Eventually, the established HPLC–DAD method was successfully applied to the simultaneous determination of polydatin, isoquercitrin, resveratrol, and nicotiflorin in the extract of herb T. hemsleyanum.

【 授权许可】

Unknown