Multi-omics analyses revealed key factors involved in fluorescent carbon-dots-regulated secondary metabolism in Tetrastigma hemsleyanum

Journal of Nanobiotechnology

Yuhui Wang¹  Xiuhua Wang¹  Jianping Zheng¹  Zhuomi Xie²  Mingjie Li²  Zhongyi Zhang²  Chuyun Yang²  Xin Peng³  Yuxiang Zhao³ 

[1] Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, 315300, Ningbo, People’s Republic of China;Fujian Agriculture and Forestry University, 350028, Fuzhou, People’s Republic of China;Ningbo Research Institute of Zhejiang University, 315100, Ningbo, People’s Republic of China;

关键词: Fluorescent carbon-dots;    Tetrastigma hemsleyanum;    Quality ingredients;    Hub genes;   

DOI  :  10.1186/s12951-022-01271-6

来源: Springer

【 摘 要 】

BackgroundLuminescent nanomaterials (LNMs), especially newly-exploited fluorescent carbon-dots (CDs), have demonstrated promising candidates for sunlight harvesting and enhanced photosynthesis efficiency of crops. However, most of the studies focus on the design and synthesis of LNMs and primary metabolism in biomass acceleration, secondary metabolism that closely associated with the quality ingredients of plants is rarely mentioned.ResultsUV-absorptive and water-soluble NIR-CDs were harvested via a facile microwave-assisted carbonization method. The effect and regulatory mechanism of NIR-CDs on the secondary metabolism and bioactive ingredients accumulation in Tetrastigma hemsleyanum were explored. A total of 191 differential secondary metabolites and 6874 differentially expressed genes were identified when the NIR-CDs were adopted for enhancing growth of T. hemsleyanum. The phenolic acids were generally improved, but the flavonoids were more likely to decrease. The pivotal differentially expressed genes were involved in biosynthesis of secondary metabolites, flavonoid biosynthesis, porphyrin and chlorophyll metabolism, etc. The gene-metabolite association was constructed and 44 hub genes highly related to quality ingredients accumulation and growth were identified, among which and the top 5 genes of the PPI network might be the key regulators.ConclusionThis research provided key regulatory genes and differentially accumulating quality ingredients under NIR-CDs-treatment, which could provide a theoretical basis for expanding the applications of nanomaterial in industrial crop agriculture.Graphical Abstract

【 授权许可】

CC BY