Locating and Characterizing a Transgene Integration Site by Nanopore Sequencing

G3: Genes, Genomes, Genetics

Ting-Jan Cho^1¹  Tatyana Pyntikova^1²  Daniel W. Bellott^1³  Peter K. Nicholls^1⁴ 

[1] Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139;Howard Hughes Medical Institute, Whitehead Institute, Cambridge, MA 02142^2;Whitehead Institute, 455 Main Street, Cambridge, MA 02142;Whitehead Institute, 455 Main Street, Cambridge, MA 02142^1

关键词: nanopore;    transgene;    germ line;    mouse;    Oct4:EGFP;   

DOI  :  10.1534/g3.119.300582

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

The introduction of foreign DNA into cells and organisms has facilitated much of modern biological research, and it promises to become equally important in clinical practice. Locating sites of foreign DNA incorporation in mammalian genomes has proven burdensome, so the genomic location of most transgenes remains unknown. To address this challenge, we applied nanopore sequencing in search of the site of integration of Tg(Pou5f1-EGFP)^2Mnn (also known as Oct4:EGFP ), a widely used fluorescent reporter in mouse germ line research. Using this nanopore-based approach, we identified the site of Oct4:EGFP transgene integration near the telomere of Chromosome 9. This methodology simultaneously yielded an estimate of transgene copy number, provided direct evidence of transgene inversions, revealed contaminating E. coli genomic DNA within the transgene array, validated the integrity of neighboring genes, and enabled definitive genotyping. We suggest that such an approach provides a rapid, cost-effective method for identifying and analyzing transgene integration sites.

【 授权许可】

CC BY