Culturable fungal diversity and cellulase production by mixed culture Aspergillus fungi from Sanya mangrove

The Journal of General and Applied Microbiology

Manzoor Ahmad¹  Liyun Lin²  Juan Ling³  Siquan Zeng⁴ 

[1] Guangdong Provincial Key Laboratory of Applied Marine Biology;Guangdong Provincial Key Laboratory of Applied Marine Biology,Tropical Marine Biological Research Station, Chinese Academy of Sciences,Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences;Guangdong Provincial Key Laboratory of Applied Marine Biology,University of Chinese Academy of Sciences;Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences

关键词: Aspergillus;    cellulase;    fungi;    mangrove;    mixed culture;   

DOI  :  10.2323/jgam.2018.02.004

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Fungi are the most suitable cellulase producers attributing to its ability to produce a complete cellulase system. 33 Genus, 175 Species fungi were isolated from Sanya mangrove, Hainan, China. Using congo red cellulose (CMC) medium, five fungi of cellulose-degrading were selected for further study. Molecular biology and morphological identification showed that all of these five fungi belong to Aspergillus fungi. The cellulase produced by these fungi were monitored during liquid state fermentation. The optimum conditions study for enzyme production illustrated that the highest activities appeared at pH 3.0, 35°C after fermentation for 3 days. Beyond that, the enzyme activity of mixed fungi is 11–26% higher than pure. The study demonstrated that mixed culture improved the hydrolysis of fungi cellulase.

【 授权许可】

Unknown