Isolation and purification of two tyrosinase inhibitors from camellia pollen by high-speed counter-current chromatography

Acta Chromatographicae

Yang Y.-F.¹ 

[1] College of Food and Bioengineering, Jimei University, Xiamen, Fujian 361021

关键词: Camellia pollen;    high-speed counter-current chromatography;    levulinic acid;    5-hydroxymethylfurfural;    tyrosinase;   

DOI  :  10.1556/1326.2017.29407

学科分类：化学（综合）

来源: Akademiai Kiado Rt.

【 摘 要 】

Bee pollen is a health food with a wide range of nutritional and therapeutic properties. However, the bioactive compounds of bee pollen have not been extensively revealed due to low efficacy in separation. High-speed counter-current chromatography (HSCCC) and solvent extraction were applied to separate tyrosinase inhibitors from camellia pollen in this study. The camellia pollen extracts prepared with petroleum ether, ethyl acetate, and n-BuOH have tyrosinase inhibitory activity. Acidic hydrolysis could promote the tyrosinase inhibitory activity of crude sample. Three fractions with tyrosinase inhibitory activity were separated from the hydrolysate by a one-step HSCCC procedure. Among the fractions, two chemicals were sufficiently purified and identified to be levulinic acid (LA) and 5-hydroxymethylfurfural (5-HMF). The recovery was 0.80 g kg−1 pollen for LA and 1.75 g kg−1 pollen for 5-HMF; and their purity was all over 98%. The study demonstrates that HSCCC method is powerful for preparative separation of tyrosinase inhibitors from camellia pollen.

【 授权许可】

Unknown