Preparative Isolation and Purification of Lignans from Justicia procumbens Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode

Molecules

Peijuan Zhou¹  Qijun Luo¹  Lijian Ding²  Fang Fang²  Ye Yuan¹  Juanjuan Chen²  Jinrong Zhang²  Haixiao Jin¹  Shan He² 

[1] Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China; E-Mails:;Laboratory of Marine Natural Products, School of Marine Sciences, Ningbo University, Ningbo 315211, China; E-Mails:

关键词: Justicia procumbens;    lignans;    high-speed counter-current chromatography;    preparative separation;    stepwise elution mode;   

DOI  :  10.3390/molecules20047048

来源: mdpi

【 摘 要 】

Lignans, which are recognized as main constituents in Justicia procumbens, have attracted considerable attention due to their pharmacological activities, including antitumor, anti-hepatitic, cytotoxic, anti-microbial, and anti-virus properties. Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four lignans (justicidin B (1), justicidin A (2), 6'-hydroxyjusticidin C (3) and lignan J₁ (4)) from J. procumbens using stepwise elution with a pair of two-phase solvent systems composed of n-hexane–ethyl acetate–methanol–water at (1.3:1:1.3:1, v/v) and (2.5:1:2.5:1, v/v). The preparative HSCCC separation was performed on 300 mg of crude sample yielding compounds 1 (19.7 mg), 2 (9.86 mg), 3 (11.26 mg), and 4 (2.54 mg) in a one-step separation, with purities over 95% as determined by HPLC. The structures of these compounds were identified by MS, ¹H-NMR and ¹³C-NMR. This is the first report on the application of HSCCC to the efficient separation of lignans from J. procumbens.

【 授权许可】

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