Inhibitory effect of grapefruit seed extract (GSE) on avian pathogens

The Journal of Veterinary Medical Science

Mayuko SUZUKI¹  Miyuki KOMURA² 

[1] Bar-Ilan University, Begin-Sadat Center for Strategic Studies, Ramat Gan 5290002, Israel;Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fchu-shi, Tokyo 183-8509, Japan

关键词: avian influenza virus;    enhancement of biosecurity;    grapefruit seed extract;    spraying method;   

DOI  :  10.1292/jvms.18-0754

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The inhibitory activities of grapefruit seed extract (GSE) on avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), Salmonella Infantis (SI) and Escherichia coli (EC) were evaluated. Original GSE contained 0.24% benzalkonium chloride (BZC), however, 0.0025% BZC solution could not inactivate bacteria. The activity of diluted GSE (×100, ×500 and ×1,000 with redistilled water) against selected viruses and bacteria was evaluated in this study. The GSE solutions were incubated with the pathogens over a period of time after which the remaining viruses were titrated and the bacterial colonies were counted. In the presence of organic material—5% fetal bovine serum (FBS), the test solutions were sprayed at 1 cm and 30 cm distances to test the efficacy of GSE in a spray form. Furthermore, the efficacy of GSE against bacteria on clothes was tested using non-woven cloth. GSE×100 reduced the viral titer of both AIV and NDV even in 5% FBS condition. IBDV showed high resistance to GSE. GSE×1,000 inactivated both SI and EC within 5 sec, even in the presence of 5% FBS. The disinfectant was able to maintain its efficacy in the spray form at 30 cm distance. GSE was also effective against SI and EC inoculated on fabric. GSE is a potential novel disinfectant against viruses and bacteria, effective even within a short contact time.

【 授权许可】

Unknown