MicroRNA-365 suppressed cell proliferation and migration via targeting PAX6 in glioblastoma

American Journal of Translational Research

Fei Yuan¹  Jie Liu²  Kaikun Yuan³  Hengyuan Pang⁴  Yu Tian⁵ 

[1]Department of Neurosurgery, The 2nd Affiliated Hospital, Harbin Medical University, Harbin 150086, China

[2]Harbin Medical University, Harbin 150086, China

[3]Harbin No.3 Middle School, Harbin, China

[4]Institute of Molecular Biotechnology of The Austrian Academy of Sciences, Austrian

[5]Jiamusi University, Jiamusi 154007, China

关键词: Glioblastoma;    microRNAs;    miR-365;    PAX6;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

MicroRNAs (miRNAs) act an important role in the progression of tumor. In this study, we showed that the serum expression of miR-365 was downregulated in the glioblastoma compared with in the healthy controls. We also demonstrated that miR-365 expression was downregulated in glioblastoma tissues compared with the adjacent normal tissues. Overexpression of miR-365 suppressed the glioblastoma cell proliferation and migration. Moreover, ectopic expression of miR-365 promoted the expression of Ecadherin while inhibited the expression of N-cadherin and Vimentin in U87 cell. Furthermore, we identified PAX6 as a direct target gene of miR-365 in U87 cell. Overexpression of miR-365 suppressed glioblastoma cell proliferation and migration and epithelial-to-mesenchymal transition through inhibiting PAX6 expression. These results suggested that miR-365 played a tumor suppressor in glioblastoma.

【 授权许可】

CC BY-NC