IL-22/IL-22R1 axis is involved in myocardial injury of a mouse cecal ligation and puncture model

American Journal of Translational Research

Yingying Wu¹  Heming Yang²  Ran Duan³  Yanhua Zheng⁴  Kai Feng⁵ 

[1] Department of Emergency Medicine, Changzhou Wujin Peoples Hospital Affiliated to Jiangsu University, Changzhou, China;Department of General Surgery, 306 Hospital of PLA, Beijing, China;Department of Pathology, 306 Hospital of PLA, Beijing, China;Medical School of Chinese PLA, Beijing, China;Special Medical Center, 306 Hospital of PLA, Beijing, China

关键词: Cecal ligation and puncture;    sepsis;    mouse;    IL-22;    IL-22R1;    IL-22BP;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Myocardial depression is a hallmark of severe sepsis, which may result from a complex interplay among several factors. However, the mechanisms are still unclear yet. In this study, we aimed to explore if IL-22/IL-22R1 axis plays a role in the myocardial injury during sepsis. A cecal ligation and puncture (CLP) mouse model was established to explore the histopathological changes and to analyze the role of IL-22/IL-22R1 axis in myocardial injury during the process of sepsis. Histopathologically, myocardial injury was apparently observed with the progress of sepsis but it was improved at 72 h after surgery. On the contrary, the heart tissue in the sham group revealed injury at a limited degree at the first 8 h after surgery and then restored to normal. Results from immunohistochemical study and real-time qPCR showed that IL-22, IL-22R1 and IL-22BP had different changing trends in the progress of sepsis at both protein and mRNA levels. The expression of IL-22R1 and IL-22BP was markedly induced after CLP modeling (P < 0.01), while that of IL-22 was sharply reduced in both groups (P < 0.01). The differences in the expression of IL-22, IL-22R1 and IL-22BP between the sham and CLP groups were significant only at 72 h after surgery (P < 0.05) but not at the other time points (P > 0.05). In conclusion, IL-22/IL-22R1 axis is involved and may have a potential immunoprotective role in the cardiac tissue repair, but the immunoprotection on the cardiac tissue of CLP mice was remarkably damaged in the progress of sepsis and even in the recovery phase.

【 授权许可】

CC BY-NC