Silk Fibroin Pads for Whole Blood Glucose Determination

Proceedings

Cenis, J. L.¹  Márquez, Augusto²  Aznar-Cervantes, Salvador D.³  Domínguez, Carlos⁴ 

[1] Author to whom correspondence should be addressed.;Institut de Microelectrònica de Barcelona, (IMB-CNM, CSIC), 08193 Bellaterra (Barcelona), Spain;Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), 30150 La Alberca (Murcia), Spain;Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018

关键词: optical biosensor;    silk fibroin;    durable biosensor;    enzyme matrix;    green chemistry;   

DOI  :  10.3390/proceedings2130886

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

In this work, we present a durable and miniaturized photonic lab on a chip (PhLOC) integrating functionalized silk fibroin pads for optical glucose quantification in whole blood samples. The PhLOC consists of a poly(methyl methacrylate) (PMMA) disposable structure for the pad holding and a coupling system for commercial optic fibers connection. The silk matrix, together with the PMMA design, has the capability to separate the plasma from the cell fraction of whole blood by plasma diffusion into the silk, minimizing the absorbance interferences from the hemoglobin of the erythrocytes. Then, the enzymes present in the matrix react with the glucose in the sample and produce a color change in an amplified process where the analyte—mediator—matrix interacts sequentially, increasing the common signal from the oxidized mediator (2 fold). The sensor works in the adequate linear range to distinguish between healthy and unhealthy glucose levels (0 to 12 mM), with a sensitivity of 0.084 a.u. mM−1, a limit of detection of 0.18 mM and a limit of quantification of 1.44 mM. Furthermore, durability of the sensor is remarkable, maintaining its response unchangeable during the first 7 months of tests.

【 授权许可】

CC BY