A Guided Mode Resonance Aptasensor for Thrombin Detection

Sensors

Sheng-Fu Lin²  Ting-Jou Ding²  Jen-Tsai Liu¹  Chien-Chieh Lee³  Tsung-Hsun Yang²  Wen-Yih Chen¹ 

[1] Department of Chemical and Materials Engineering, National Central University, Jhongli 32001, Taiwan; E-Mail:;Department of Optics and Photonics, National Central University, Jhongli 32001, Taiwan; E-Mails:;Optical Sciences Center, National Central University, Jhongli 32001, Taiwan; E-Mail:

关键词: aptamer;    optical biosensor;    guided mode resonance;    thrombin;    affinity constant;   

DOI  :  10.3390/s110908953

来源: mdpi

【 摘 要 】

Recent developments in aptamers have led to their widespread use in analytical and diagnostic applications, particularly for biosensing. Previous studies have combined aptamers as ligands with various sensors for numerous applications. However, merging the aptamer developments with guided mode resonance (GMR) devices has not been attempted. This study reports an aptasensor based home built GMR device. The 29-mer thrombin aptamer was immobilized on the surface of a GMR device as a recognizing ligand for thrombin detection. The sensitivity reported in this first trial study is 0.04 nm/μM for thrombin detection in the concentration range from 0.25 to 1 μM and the limit of detection (LOD) is 0.19 μM. Furthermore, the binding affinity constant (K_a) measured is in the range of 10⁶ M⁻¹. The investigation has demonstrated that such a GMR aptasensor has the required sensitivity for the real time, label-free, in situ detection of thrombin and provides kinetic information related to the binding.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.