ELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle

The Journal of Veterinary Medical Science

Rodrigo Nestor ETGES¹  Miladin KOSTOVIC²  Kláudia Santos GONÇALVES JORGE³  Márcio Roberto SILVA⁴  Ingrid Ieda Fernando de SOUZA⁵  Rudielle de Arruda RODRIGUES⁶  Walter LILENBAUM⁷  Carlos Eugênio Soto VIDAL⁸ 

[1] Ellie Lab., Germantown, WI 53022, U.S.A.;Embrapa Beef Cattle, Campo Grande, MS 79106-550, Brazil;Embrapa Dairy Cattle, Juiz de Fora, MG 36038-330, Brazil;Laboratory of Veterinary Bacteriology, Fluminense Federal University, Niterói, RJ 24. 210-130, Brazil;Postgraduate Program in Biotechnology and Biodiversity of the Central Western Region, Federal University of Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil;Postgraduate Program in Veterinary Sciences, School of Veterinary Medicine (FAMEZ), Federal University of Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil;Regional Technical Unit for Agriculture, Livestock and Supply of Santa Maria (UTRA), Ministry of Agriculture, Livestock and Supply, Santa Maria, RS 97050-500, Brazil;Secretary of Agriculture, Livestock and Irrigation (SEAPI), State Government of Rio Grande do Sul, Porto Alegre, RS 90150-900, Brazil

关键词: bovine tuberculosis;    comparative intradermal tuberculin test;    ELISA;    Mycobacterium bovis;   

DOI  :  10.1292/jvms.18-0364

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M. bovis culturing and PCR. Paired comparisons of all data (n=92) demonstrated that ELISA and LST results compared to the culturing results did not present significant differences (P=0.27 on McNemar’s test and P=0.12 on Fisher’s exact test, respectively). Using culturing as the gold standard, the sensitivity and specificity of ELISA were 79.5% (95% CI: 64.5–89.2%) and 75.5% (95% CI: 62.4–85.1%), respectively, whereas LST demonstrated 100% sensitivity (95% CI: 91.03–100%) and 92.5% specificity (95% CI: 82.1–97.0%). The ELISA results did not reveal significant differences in relation to the LST results (P>0.99 on Fisher’s exact test). Using the latter as the gold standard, the sensitivity and specificity of ELISA were 79.1% (95% CI: 64.8–88.6%) and 79.6% (95% CI: 66.4–88.5%), respectively. The use of ELISA with the recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen complements the diagnostic coverage provided by CITT and increases the removal of infected animals from herds.

【 授权许可】

Unknown