Voided volumes predict degree of partial bladder outlet obstruction in a murine model

American Journal of Clinical and Experimental Urology

Mehul S Patel¹  Paulette M Hausner²  Nicholas M Tassone³  Megan Y Devine⁴  Belinda Li⁵ 

[1] Department of Biology, DePaul University, 1110 W. Belden Ave., Chicago, IL 60614, USA;Department of Biology, Loyola University, 1032 W. Sheridan Rd., Chicago, IL 60660, USA;Department of Pediatric Urology, Ann Robert H. Lurie Childrens Hospital of Chicago, 225 E. Chicago Ave., Chicago, IL 60611, USA;Department of Urology, Feinberg School of Medicine, Northwestern University, 303 E. Chicago Ave., 16-703, Chicago, IL 60611, USA;Department of Urology, Loyola University Health System, 2160 S. First Ave., Maywood, IL 60153, USA

关键词: Bladder;    bladder obstruction;    partial bladder outlet obstruction;    obstructive uropathy;    posterior urethral valves;    VSOP;    surgical and invasive medical procedures;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

The partial bladder outlet obstruction animal model (pBOO) is commonly used as a model for obstructive uropathy. Unfortunately, pBOO demonstrates variable degrees of obstruction requiring bladder weight (BW) or urodynamic studies to determine true obstruction. Our objective is to identify extent of obstruction by correlating early post-operative Void Stains on Paper (VSOP) assays with ultimate BW in mice. pBOO was performed on 32 mice 1- and 4-week VSOPs were quantified for mean voided volume (mVV). At 4 weeks, bladders were harvested and weighed. Correlation was evaluated through bivariate kernel density estimation and a Pearson correlation coefficient (SAS). Single variable histogram of the data established groups based on BWs and mVV. mVV’s and bladder weights within group pairings were averaged and plotted to render a non-linear regression model. A significant correlation was found between 1-week mVVs and 4-week BWs upon bivariate analysis with a correlation coefficient of -0.758 (p = 0.0294). A non-linear regression of plotted data defined a statistically significant fit equation correlating 1-week mVV to 4-week BW. We demonstrate a novel method for forecasting degree of obstruction in pBOO based on 1-week post-operative VSOP mVV.

【 授权许可】

CC BY-NC