Segmentation of Total Cell Area in Brightfield Microscopy Images

Methods and Protocols

Čepa, Martin¹ 

[1] Contipro a.s., Dolní Dobrouč 401, Dolní Dobrouč 561 02, Czech Republic

关键词: brightfield segmentation;    microscopy;    ImageJ;    Fiji;    image analysis;    cells;   

DOI  :  10.3390/mps1040043

学科分类：生物技术

来源: mdpi

【 摘 要 】

Segmentation is one of the most important steps in microscopy image analysis. Unfortunately, most of the methods use fluorescence images for this task, which is not suitable for analysis that requires a knowledge of area occupied by cells and an experimental design that does not allow necessary labeling. In this protocol, we present a simple method, based on edge detection and morphological operations, that separates total area occupied by cells from the background using only brightfield channel image. The resulting segmented picture can be further used as a mask for fluorescence quantification and other analyses. The whole procedure is carried out in open source software Fiji.

【 授权许可】

CC BY