3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells

Journal of Biological Research

L. Andrew Staehelin¹  Soren Mogelsvang²  Bryon S. Donohoe³  Dana E. Martinez⁴  Zachary R. Gergely⁵  Rachel Herder⁶ 

[1]Rosati

[2]Exxel Pharma, Inc

[3]National Renewable Energy Laboratory

[4]Universidad Nacional de La Plata–CONICET CC 327

[5]University of Colorado at Boulder

[6]Wilson Sonsini Goodrich &

关键词: Venus flytrap;    Golgi;    trans Golgi network;    Endoplasmic reticulum;    Transmission electron microscopy;    Electron tomography;   

DOI  :  10.1186/s40709-018-0086-2

学科分类：生物科学（综合）

来源: Springer

【 摘 要 】

BackgroundThe insect-trapping leaves of Dionaea muscipula provide a model for studying the secretory pathway of an inducible plant secretory system. The leaf glands were induced with bovine serum albumin to secrete proteases that were characterized via zymogram activity gels over a 6-day period. The accompanying morphological changes of the endoplasmic reticulum (ER) and Golgi were analyzed using 3D electron tomography of glands preserved by high-pressure freezing/freeze substitution methods.

【 授权许可】

Free