Tris reversibly inhibits secretion of albumin and α‐1‐antitrypsin at different sites

FEBS Letters

Judah, Jacob D.¹  Quinn, Paul¹ 

[1] Department of Physiology, University College London, University Street, London WC1E 6JJ, UK

关键词: Tris;    Albumin;    α-1 Antitrypsin;    Golgi;    Endoplasmic reticulum;    (Rat hepatocyte);   

DOI  :  10.1016/0014-5793(90)81415-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated the effect of the weak base Tris on the processing and secretion of albumin and α-1 antitrypsin by hepatocytes in culture. We show that the secretion of both proteins is 90% inhibited by 30 mM Tris. The post-synthetic processing of both proteins is inhibited to the same extent. These effects are completely reversible. Cell fractionation indicates that albumin accumulates in the Golgi, whereas α-1 antitrypsin fails to leave the endoplasmic reticulum.

【 授权许可】

Unknown