Microbial mediated preparation, characterization and optimization of gold nanoparticles

Revista de microbiologia

Honary, Soheila¹  Barabadi, Hamed²  Mohammadi, Milad Ali³  Ebrahimi, Pouneh⁴ 

[1] Golestan University, Gorgan, Iran;Mazandaran University of Medical Sciences, Sari, Iran;Shaheed Beheshti University of Medical Sciences, Tehran, Iran;Tehran University of Medical Sciences, Tehran, Iran

关键词: biosynthesis;    optimization;    gold nanoparticles;    box-behnken design;    Penicillium crustosum.;   

DOI  :  10.1590/S1517-83822014000400046

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The need for eco-friendly and cost effective methods for nanoparticles synthesis is developing interest in biological approaches which are free from the use of toxic chemicals as byproducts. This study aimed to biosynthesize and optimize the size of gold nanoparticles which produced by biotechnological method using Penicillium crustosum isolated from soil. Initially, Penicillium crustosum was grown in fluid czapek dox broth on shaker at 28 ºC and 200 rpm for ten days and then the supernatant was separated from the mycelia to convert AuCl4 solution into gold nanoparticles. The synthesized nanoparticles in the optimum conditions were formed with fairly well-defined dimensions and good monodispersity. The characterizations were done by using different methods (UV-Visible Spectroscopy, Fluorescence, FT-IR, AFM (Atomic Force Microscopy) and DLS (Dynamic Light Scattering). The bioconversion was optimized by Box-Behnken experimental design. The results show that the effective factors in this process were concentration of AuCl4, pH of medium and temperature of shaker incubator. The R2 value was calculated to be 0.9999 indicating the accuracy and ability of the polynomial model. It can be concluded that the use of multivariate analysis facilitated to find out the optimum conditions for the biosynthesis of gold nanoparticles induced by Penicillium crustosum in a time and cost effective process. The current approach suggested that rapid synthesis of gold nanoparticles would be suitable for developing a biological process for mass scale production of formulations.

【 授权许可】

CC BY-NC