Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from rhizosphere of Valeriana jatamansi and its growth promotional effect

Revista de microbiologia

Walia, Abhishek¹  Mehta, Preeti²  Shirkot, Chand Karan³  Mahajan, Rishi⁴  Guleria, Shiwani⁵  Balgir, Praveen P.⁶  Chauhan, Anjali⁷ 

[1] DAV University, Jalandhar, India;DBT-IOC Centre for Bioenergy Research, Faridabad, India;Dr Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, India;Jaypee University of Information Technology, Waknaghat, India;Lovely Professional University, Jalandhar, India;Punjabi University Patiala, Patiala, India;University of Horticulture and Forestry, Nauni, India

关键词: Valeriana jatamansi;    Phosphate solubilization;    Nitrogen fixation;    Glucose dehydrogenase;    Aneurinibacillus aneurinilyticus;    PGPR.;   

DOI  :  10.1016/j.bjm.2016.12.001

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260 mg/L), nitrogen fixation (202.91 nmol ethylene mL-1 h-1), indole-3-acetic acid (IAA) (8.1 µg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1.34%) and oxalic acids. However, genetic evidences for nitrogen fixation and phosphate solubilization by organic acid production have been reported first time for A. aneurinilyticus strain CKMV1. A unique combination of glucose dehydrogenase (gdh) gene and pyrroloquinoline quinone synthase (pqq) gene, a cofactor of gdh involved in phosphate solubilization has been elucidated. Nitrogenase (nif H) gene for nitrogen fixation was reported from A. aneurinilyticus. It was notable that isolate CKMV1 exhibited highest antifungal against Sclerotium rolfsii (93.58%) followed by Fusarium oxysporum (64.3%), Dematophora necatrix (52.71%), Rhizoctonia solani (91.58%), Alternaria sp. (71.08%) and Phytophthora sp. (71.37%). Remarkable increase was observed in seed germination (27.07%), shoot length (42.33%), root length (52.6%), shoot dry weight (62.01%) and root dry weight (45.7%) along with NPK (0.74, 0.36, 1.82%) content of tomato under net house condition. Isolate CKMV1 possessed traits related to plant growth promotion, therefore, could be a potential candidate for the development of biofertiliser or biocontrol agent and this is the first study to include the Aneurinibacillus as PGPR.

【 授权许可】

CC BY-NC