Helicobacter Pylori Causes Oxidative Stress and Apoptosis in DNA Double Strand Break Repair Inhibited Human Gastric Adenocarcinoma Cells

Proceedings

Koçer-Gümüşel, Belma¹  Özkemahlı, Gizem²  Sur, Ünzile³  Oral, Didem⁴  Akyön, Yakut⁵  Erkekoğlu, Pınar⁶ 

[1] Author to whom correspondence should be addressed.;Department of Medical Microbiology, Faculty of Medicine, Hacettepe University, 06100 Sıhhiye Ankara, Turkey;Department of Toxicology, Faculty of Pharmacy, Erzincan Binali Yildirim University, 24100 Erzincan, Turkey;Department of Toxicology, Faculty of Pharmacy, Hacettepe University, 06100 Sıhhiye Ankara, Turkey;Department of Toxicology, Faculty of Pharmacy, Lokman Hekim University, Çankaya 06510 Ankara, Turkey;Presented at the 2nd International Cell Death Research Congress, Izmir, Turkey, 1–4 November 2018.

关键词: Helicobacter pylori;    AGS cells;    gastric carcinoma;    reactive oxygen species;    apoptosis;   

DOI  :  10.3390/proceedings2251544

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Helicobacter pylori, a helix-shaped gram-negative microaerophilic bacteria has been classified as a human carcinogen (Group I carcinogen) by the International Agency for Research on Cancer (IARC) on the basis of numerous animal and epidemiological studies. Chronic Helicobacter pylori infection can cause lead to high levels of intracellular reactive oxygen species (ROS) and genomic instability both directly by genetic and/or epigenetic pathways. It is known that high levels of intracellular ROS can trigger apoptosis and induce DSBs. In this study, we aimed to investigate intracellular ROS levels and apoptotic effects of Helicobacter pylori infection on AGS cells in the presence of a DSB repair inhibitor [8-(4-dibenzothienyl)-2-(4-morpholinyl)-4H-1-benzopyran-4-one, Nu7441]. After incubation of AGS cells with different multiplicities of infection (MOIs; 25, 50, 75, 100, 200 and 400), we observed that Helicobacter pylori causes MOI-dependent cytotoxicity and intracellular ROS levels significantly increased in all study groups vs. control. Both caspase 3 and caspase 8 levels were higher all of the study group when compared to control group. In HP+Nu7441 group, caspase 8 levels 3.5 times higher vs. control; indicating unrepaired DSBs in Helicobacter pylori infection can highly be induce apoptosis in AGS cells. Other cell death mechanisms, including autophagy, should be studies in order to fully understand the cytotoxicity mechanisms of the bacterium. Moreover, we can state that in organisms with unrepaired DSBs or with high DSB levels, Helicobacter pylori can cause more oppressive adverse effects, the importance of which should be elucidated with mechanistic studies.

【 授权许可】

CC BY