Characterization of Francisella species isolated from the cooling water of an air conditioning system

Revista de microbiologia

Gu, Quan¹  Qu, Pinghua²  Li, Xunde³ 

[1] Guangzhou Center for Disease Control and Prevention, Guangzhou, CH;University City Branch, Guangzhou, CH;University of California, US

关键词: Francisella;    air conditioning;    water.;   

DOI  :  10.1590/S1517-838246320140465

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition, these bacteria grow on cysteine-supplemented media at 20 °C to 40 °C with an optimal growth temperature of 30 °C. Analysis of 16S rRNA gene sequences revealed that these strains belong to the genus Francisella. Biochemical tests and phylogenetic and BLAST analyses of 16S rRNA, rpoB and sdhA genes indicated that one strain was very similar to Francisella philomiragia and that the other strains were identical or highly similar to the Francisella guangzhouensis sp. nov. strain 08HL01032 we previously described. Biochemical and molecular characteristics of these strains demonstrated that multiple Francisella species exist in air conditioning systems.

【 授权许可】

CC BY-NC