Qualitative and quantitative mycotoxin analyses in residual water of rice production chain by TLC and HPTLC

Química Nova

Badiale-Furlong, Eliana¹  Fagundes, Carlos Alberto¹  Garda-Buffon, Jaqueline¹  Leite, Clarice Caldeira¹ 

关键词: method validation;    mycotoxins;    water.;   

DOI  :  10.1590/S0100-40422012001000013

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This study validated a simple and applied method for determining mycotoxins aflatoxin B1, aflatoxin B2, ochratoxin A, zearalenone and deoxynivalenol, in water from the rice production chain. Five solvent combinations for extraction were tested, with quantification performed by TLC/HPTLC and confirmation by LC-MS/MS. Mycotoxins in water from field and rice industries were evaluated. Mycotoxin recovery levels were around 90%. Two samples from rice parboiling waste were contaminated (deoxynivalenol/aflatoxin B1, 110/9 ng mL-1; and deoxynivalenol, 100 ng mL-1). Zearalenone, deoxynivalenol and ochratoxin A (36, 30 and 28%) were carried to soaking water during parboiling.

【 授权许可】

Unknown