Heat challenge influences serum metabolites concentrations and liver lipid metabolism in Japanese quail (Coturnix japonica)

The Journal of Veterinary Medical Science

Kento USUDA¹  Shaoxia PU² 

[1] Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan;Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan

关键词: heat challenge;    Japanese quail;    lipid metabolism;    liver;    serum metabolite;   

DOI  :  10.1292/jvms.18-0615

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

High temperature induces various metabolic disturbances in animals. However, no comprehensive information is currently available on the metabolic pathway affected by high environmental temperature. The present study examined metabolite content in the serum of heat challenged quails using metabolomic analysis. In the present study, female quails with normal laying rate at 20 weeks kept in standard condition (control group) or exposed to 34°C 4 hr per day (12:00 to 16:00 hr)(heat group) for 10 consecutive days. The metabolomic analysis identified 165 metabolites in the serum, and significant differences were observed in the serum for 7 metabolites between two groups. An analysis by MetaboAnalyst, a web-based metabolome data tool, indicate that high temperature affect ketone body metabolism, butyrate metabolism, arginine and proline metabolism. Furthermore, histological examination of liver indicates a heat challenge induced abnormal lipid metabolism. Triglyceride and cholesterol level in the liver increased, however cholesterol level decreased in the serum. Genes related to lipid metabolism significantly increased in the liver after heat challenge. The present study demonstrated that high temperature cause liver damage, thus lipid metabolic was affected. Protect liver under high temperature could be one solution for coping with high temperatures in summer.

【 授权许可】

Unknown