Recent advances in microfluidic technologies for biochemistry and molecular biology

BMB Reports

Andrew J. deMello^3¹  Dong-Ku Kang^1²  Soongwon Cho^1³  Jaebum Choo^2⁴ 

[1] Department of Biochemistry, Chungbuk National University, Cheongju 361-763, Korea^4;Department of Bionano Engineering, Hanyang University, Ansan 426-791, Korea^2;Department of Chemistry, Imperial College London, London, UK^1;Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Switzerland^3

关键词: Biosensors;   

DOI  :  

学科分类：生物化学/生物物理

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large amounts of biological chemical data into an organized database of knowledge. Microfluidic systems are ideally suited for high-throughput biochemical experimentation since they offer high analytical throughput, consume minute quantities of expensive biological reagents, exhibit superior sensitivity and functionality compared to traditional micro-array techniques and can be integrated within complex experimental work flows. A range of basic biochemical and molecular biological operations have been transferred to chip-based microfluidic formats over the last decade, including gene sequencing, emulsion PCR, immunoassays, electrophoresis, cell-based assays, expression cloning and macromolecule blotting. In this review, we highlight some of the recent advances in the application of microfluidics to biochemistry and molecular biology.

【 授权许可】

Unknown