Genetic characteristics of feral Misaki horses based on polymorphisms of microsatellites and mitochondrial DNA

The Journal of Veterinary Medical Science

Masaki TAKASU¹  Teruaki TOZAKI²  Masaru AKITA³  Hironaga KAKOI⁴  Ikuo KOBAYASHI⁵ 

[1] Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;Education Center for Food Animal Health, Gifu University (GeFAH), 1-1 Yanagido, Gifu 501-1193, Japan;Kushima City, 5550 Nishikata, Kushima, Miyazaki 888-8555, Japan;Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan;Sumiyoshi Livestock Science Station, Field Science Center, University of Miyazaki, 10100-1 Shimanouchi, Miyazaki 880-0121, Japan

关键词: conservation;    genetic characteristics;    Misaki horse;   

DOI  :  10.1292/jvms.18-0565

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

The Misaki horse is a Japanese native horse, known as the “feral horse of Cape Toi”. In this study, we acquired the genetic information to establish their studbook, and analyzed their genetic characteristics for conservation. We genotyped 32 microsatellites and a mitochondrial D-loop region in 77 Misaki horses (80.2% of the population). The average number of alleles, observed heterozygosity, and expected heterozygosity were 3.4, 0.509, and 0.497, respectively. A neighbor-joining phylogenetic tree of individuals was constructed. Moreover, the results suggested that Misaki horses experienced a bottleneck, but it was neither severe nor recent. In addition, three mitochondrial haplotypes were confirmed. Consequently, we clarified the genetic background of Misaki horses that have been resident at Cape Toi for a long time.

【 授权许可】

Unknown