Providencia in retail meats from Guangzhou, China and Osaka, Japan: prevalence, antimicrobial resistance and characterization of classes 1, 2 and 3 integrons

The Journal of Veterinary Medical Science

Qingyang LI¹  Sisi LIANG²  Huiling DI³  Lei SHI⁴ 

[1] Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan;Research Institute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, China;School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China;State Key Laboratory of Food Safety Technology for Meat Products, Xiamen Yinxiang Group Co., Ltd., Xiamen 361100, China

关键词: antimicrobial resistance;    integron;    Providencia;    retail meat;   

DOI  :  10.1292/jvms.18-0037

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Bacteria of the genus Providencia are opportunistic pathogens of clinical significance due to their association with diarrhea and urinary tract infections. The present study was conducted to examine the prevalence and antimicrobial resistance of Providencia spp. in retail meats sold in Guangzhou, China and Osaka, Japan. Out of 158 meat samples including beef, pork and chicken, 67 Providencia (42%) belonging to four species viz., P. alcalifaciens, P. rustigianii, P. stuartii and P. rettgeri were isolated, and most of them were resistant to tetracycline (91%) followed by ampicillin (69%) and streptomycin (49%). Of 67 isolates, 29 (43%) were MDR, which is defined to be resistant to more than three classes of antimicrobials. No statistically significant differences were observed between Chinese and Japanese retail meat samples regarding contamination rate of Providencia spp. as well as frequency of the antimicrobial resistance of the isolates including MDR. Class 1 and/or class 2 integrons were detected in six of the eight isolates that were resistant to more than 4 antimicrobials, however none of the isolates harbored class 3 integron. A P. rustigianii harboring the blaOXA-10 gene was isolated, which is the first report of Providencia with blaOXA-10 gene of food origin. These data suggest that retail meats in China and Japan are substantially contaminated with Providencia spp., which displayed a high frequency of antimicrobial resistance, and establishing the surveillance of Providencia spp., especially antimicrobial resistant one, in retail meats is imperative.

【 授权许可】

Unknown