The Effect of Different Titers of Blood-Grouping Reagents on Proliferation and Apoptosis of Bone Marrow Derived Mesenchymal Stem Cells

Proceedings

Köksal, Yasin¹  Aydoğdu, Mücahit²  Özgüner, Habibe Meltem³  Ünver, Merve⁴ 

[1] Ankara Child Health and Disease Hematology Oncology Training and Research Hospital, 06080 Ankara, Turkey;Author to whom correspondence should be addressed.;Department of Histology and Embryology, Faculty of Medicine, Ankara Yıldırım Beyazıt University, 06800 Ankara, Turkey;Presented at the 2nd International Cell Death Research Congress, Izmir, Turkey, 1–4 November 2018

关键词: Hematopoetic Stem Cell Transplantation (HSCT);    Graft Versus Host Disease (GVHD);    Mesenchymal Stem Cells (MSCs);    apoptosis;   

DOI  :  10.3390/proceedings2251535

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Hematopoetic Stem Cell Transplantation (HSCT) has benefical effects for the treatment of leukemia but one of the adverse effect is Graft versus Host Disease (GVHD) that causes high mortality and morbidity. Mesenchymal Stem Cells (MSCs) are one of the promising tools which are used for the treatment of steroid refractory GVHD. The success of cellular therapies depends on so many factors associated with the donor and host characteristics. We designed a study concerning that blood group reagents at different titres can affect the survival of MSCS in blood as they were injected intravenously when used for the treatment of GVHD. We concluded that proliferation rates of MSCs are decreasing in relation with the high reagent titres. Flow-cytometric analysis also revealed that high titers of reagent in culture medium could lead to the induction of apoptotic pathways in MSCs. For individualized treatment strategies, donor and host characteristics must be taken in to consideration in details and experimental studies in cell culture laboratories could be essential to gain insight into clinical trials.

【 授权许可】

CC BY