Apoptosis and expression of argyrophilic nucleolus organizer regions in epithelial neoplasms of the larynx

Revista Brasileira de Otorrinolaringologia

Ribeiro, Christiana Vargas¹  Andrade Filho, José de Souza²  Vasconcelos, Anilton Cesar³ 

[1] Faculdade e Escola Técnica Novo Rumo, Belo Horizonte, Brazil;Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;University of California, California, USA

关键词: Apoptosis;    Larynx;    Squamous cell carcinoma;    Nucleolus organizer region;    Papilloma.;   

DOI  :  10.1016/j.bjorl.2014.12.003

学科分类：医学（综合）

来源: Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervicofacial

【 摘 要 】

INTRODUCTION: Occurrence of apoptosis and expression of proliferative markers are powerful tools to establish a prognosis in the follow-up of cancer.OBJECTIVE: To evaluate the growth fraction in papillomas and laryngeal squamous cell carcinomas with three degrees of differentiation through apoptosis and the expression of nucleolus organizer regions.METHODS: Retrospective study from which paraffin material was submitted to microtomy and hematoxylin-eosin and silver staining. Stained slides were used to quantify the apoptotic index and the number of nucleolus organizer regions by morphometry.RESULTS: Apoptosis was significantly more frequent in well differentiated carcinomas and in papillomas, and a higher growth fraction of expressed nucleolus organizer regions and cells that expressed a greater than average number of nucleolus organizer regions were more frequently noted in undifferentiated carcinomas.CONCLUSIONS: Thus, it was possible to verify that a high apoptotic index was associated with a lower chance of tumor differentiation in carcinomas, while a greater number of total nucleolus organizer regions, cells expressing nucleolus organizer regions above average and a higher growth fraction were associated with greater likelihood of abnormal cell proliferation and increased tumor differentiation.

【 授权许可】

CC BY