Candida albicans: genotyping methods and clade related phenotypic characteristics

Revista de microbiologia

Moraes, Karen C.M.¹  Moreira, Leonardo M.²  Lyon, Juliana P.³  Aimbire, Flávio⁴ 

[1] Universidade Federal de Minas Gerais, Belo Horizonte, Brasil;Universidade Federal de Ouro Preto, Ouro Preto, Brasil;Universidade Federal de São João Del Rei, São João Del-Rei, Brasil;Universidade do Vale do Paraíba, São José dos Campos, Brasil

关键词: Candida albicans;    clonal reproduction;    microevolution;    genotyping systems;    phenotypic characteristics.;   

DOI  :  10.1590/S1517-83822010000400003

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Several molecular methods, such as Southern blotting hybridization, Multilocus Sequence Typing, and DNA microsatellite analysis, have been employed to genotype Candida albicans. The genotype analysis allows to group strains in clades, that is, a group composed of one ancestor and its descendants. These genotype studies demonstrate that clades distribution is influenced by geographic area as well as that antifungal resistance is associated with particular clades. These findings suggested that C. albicans reproduces mainly in a clonal manner, with certain degree of DNA microevolution. Additionally, virulence factors and site of isolation have also been associated with clade specificity. The present article is a brief review about the methods used for Candida genotyping and the correlated clade systems established. Special emphasis is given to Ca3 hybridization, MLST, and Microsatellites. The present work is also focused on the phenotypic and physiological traits associated with Candida clades.

【 授权许可】

CC BY-NC