In vitro Evaluation of the Effects of Fluconazole and Nano-Fluconazole on Aspergillus flavus and A. fumigatus Isolates

Jundishapur Journal of Microbiology

Seyed Jamal Hashemi¹  Mohammad Reza Sarrafha² 

[1] Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran;Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, IR Iran

关键词: In vitro Evaluation of the Effects of Fluconazole and Nano-Fluconazole on Aspergillus flavus;    A. fumigatus Isolates;    Antifungal Agent;    Fluconazole;    Aspergillus flavus;    Aspergillus fumigatus;   

DOI  :  10.5812/jjm.57875

学科分类：微生物学和免疫学

来源: Jundishapur Journal of Microbiology

【 摘 要 】

Increasing azole resistance among Aspergillus strains was observed over the last decade. For this reason, viable alternatives for the current medicines are required.The current study aimed at producing fluconazole-loaded liposomal nanoparticles and comparing in vitro antifungal activity of fluconazole and nano-fluconazole on Aspergillus flavus and A. fumigatus species isolated from patients and poultry.Fifty isolates of A. flavus and A. fumigatus were collected from visceral and superficial fungal lesions of humans as well as pulmonary fungal infection of poultry. Liposomal nanoparticles were prepared by thin-film hydration method, using soybean lecithin, cholesterol, and fluconazole in a ratio of 10:1:1. The nanoparticles were analyzed in terms of size, zeta potential, and morphology. Antifungal susceptibility testing was carried out to examine and evaluate the minimum inhibitory concentration (MIC) of fluconazole and nano-fluconazole against Aspergillus species by the standard method of broth microdilution as described in CLSI (the clinical and laboratory standards institute) -M38A2.The particle size of liposomes containing fluconazole was 88.9 ± 12.1 nm and its zeta potential was -20.12 ± 1.88 mv. Nano-liposomes containing fluconazole significantly reduced the MIC against A. flavus (P = 0.0005) and A. fumigatus (P < 0.0001).The results of the current in vitro study showed that nano-fluconazole has better antifungal effects than the common form of drug on A. flavus and A. fumigatus species. The current study showed the antifungal activity of nano-drugs.

【 授权许可】

CC BY-NC