Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis

Biological research: BR

Bongkoch Turathum¹  Morakot Sroyraya²  Chinarat Changsangfa³  Sittiruk Roytrakul⁴ 

[1] Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand;Department of Anatomy, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand;Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand;National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, Thailand

关键词: Proteome;    Cat oocyte;    Cryopreservation;    Vitrification;   

DOI  :  10.1186/s40659-018-0176-5

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

The domestic cat serves as an animal model for assisted reproductive studies of endangered felid species. To date, there are no data on the protein alterations following cryopreservation of oocytes in felid family. Immature (germinal vesicle) domestic cat oocytes were vitrified in the vitrification solution containing 35% ethylene glycol, 20% DMSO and 0.5 mM sucrose. The vitrified-warmed oocytes were matured (metaphase II) in vitro and subjected to proteomic analysis using 1DE SDS-PAGE prefractionation combined with LC–MS/MS. A total of 1712 proteins were identified in in vitro matured oocytes. Of the 1712 proteins, 1454 proteins were found in both groups, whereas, 258 proteins were differentially expressed between control and vitrified-warmed groups. In vitrified-warmed oocytes, the missing proteins were membrane and nuclear proteins; whereas, apoptosis and DNA repair proteins were overrepresented. The identified missing and overexpressed proteins in vitrified-warmed oocytes represent potential markers of cryoinjuries and the developmental pathways of oocytes. The findings of differential expressed proteins may contribute to effective ways of proteome analysis of oocyte/embryo quality in order to assess safety of cryopreservation in felid species.

【 授权许可】

CC BY