Cucumber mosaic virus infecting ‘Cavendish’ banana in Argentina

Australasian Plant Disease Notes

Claudia Nome¹  Margarita Jaramillo Zapata²  Dariel Cabrera Mederos³  Carolina Torres⁴  José Villarreal Filipovich⁵ 

[1] Centro de Validación de Tecnologías Agropecuarias (CEDEVA Misión Tacaaglé);Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET);Instituto Nacional de Tecnología Agropecuaria (INTA);Universidad Central ‘Marta Abreu’ de Las Villas;Universidad de San Pablo-T

关键词: Cucumber mosaic virus;    Banana crops;    Sequence analysis;   

DOI  :  10.1007/s13314-018-0322-5

学科分类：农业科学（综合）

来源: Springer

【 摘 要 】

Banana plants (Musa sp.) showing severe mosaic symptoms in leaves were observed in Laguna Naineck, Formosa, Argentina. Electron microscopy observations of leaf dip preparations revealed isometric particles ca. 30 nm in diameter, which were identified as cucumber mosaic virus (CMV) by enzyme-linked immunosorbent assay (ELISA) test and reverse transcription-polymerase chain reaction (RT-PCR). CMV was mechanically transmitted to healthy Nicotiana glutinosa plants. Sequence analysis of coat protein gene showed highest nucleotides identity with other CMV isolates from United States of America, Serbia, Russia, Democratic Republic of Congo, Iran and Brazil, ranging between 96.9–98.3%. Phylogenetic analysis indicated that the virus isolate named Laguna Naineck (MH716245) belongs to the CMV group IA. To our knowledge, this is the first report of CMV in banana in Argentina. Moreover, constitutes the first Argentine CMV isolate available in the international nucleotide sequence databases.

【 授权许可】

CC BY