Cytological multimarker screening using BMCyt test in waterpipe smokers: an integrative study of cell damage, toxicological and cancer risk

Journal of genetics

MARUHEN A. D. SILVEIRA^1¹ 

[1] Centre of Biological Sciences and Health, College of Biological Sciences, State University of West Paraná (UNIOESTE), Universitária Street, 2069, Cascavel, Paraná 85819-110, Brazil^1

关键词: cytogenetic biomarkers;    cancer risk;    DNA damage;    micronuclei;    waterpipe smokers;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Waterpipe smoking is an ancient method of tobacco smoking practiced worldwide. There is a common belief that waterpipe smoking is a safer alternative to cigarette, but many studies showed that some toxicants were associated with cancer risk,significantly higher in waterpipe smoking. Thus, this study aimed to evaluate the status of waterpipe smoker’s buccal cells and its cancer risk using the buccal micronucleus cytome test. Forty waterpipe smokers (noncigarette smokers) were recruited and paired by gender, age and alcoholic habits with 40 control subjects. One-thousand cells from each individual were analysed and the numberof pyknotic cells (PYC), karyolitic cells (KYL), karyorrhetic cells (KHC), condensed chromatin (CC), binucleated cells (BN), basal cells (BC), nuclear buds (NBUD) and differentiated cells (DIFF) were counted. Additionally, 2000 differentiated cells were analysedcountingmicronucleated cells (MNi) and nuclear buds. We observed an increasing P< 0.05 in all waterpipe smoker's cell parameters, except DIFF (fold-decrease). Only CC showed no differences between groups. The interference in the cell cycle plus DNA damage observed in this study could be responsible for the high number of damaged cells and in death process, showing the importance ofour study and the high risk in waterpipe smoking

【 授权许可】

CC BY