Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H2O2 Measurements

Proceedings

Kühl, Michael¹  Moßhammer, Maria²  Koren, Klaus³ 

[1] Climate Change Cluster, University of Technology Sydney, Sydney, NSW 2007, Australia;Department of Biology, University of Copenhagen, 3000 Helsingør, Denmark;Department of Bioscience—Microbiology, University of Aarhus, 8000 Aarhus, Denmark

关键词: hydrogen peroxide;    H2O2;    flow injection analysis;    FIA;    optical chemical sensor;    microdialysis probe;    MDP;    chemiluminescence;    extracellular;    MDP-FIA;   

DOI  :  10.3390/proceedings2130992

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

This paper describes the optimization of a published flow injection analysis system coupled with microdialysis probes (MDP-FIA) for in-situ sampling and online measurements of hydrogen peroxide (H2O2). By modifying the commonly used Na2CO3 buffer by addition of EDTA and a changed order in reagent injection, interfering transition metals such as Fe(II) and Fe(III) are complexed and removed from the system without interfering with the chemiluminescent reaction of the used acridinium ester and H2O2. The system was then used to monitor changes in H2O2 concentration upon microwaving seawater and filtered seawater in the presence and absence of agar.

【 授权许可】

CC BY