Culture conditions influence satellite cell activation and survival of single myofibers

Basic and applied myology

Sergio Adamo¹  Anna Benedetti¹  Viviana Moresi¹  Alessandra Renzini¹  Marina Bouché¹  Leopoldo Silvestroni² 

[1] DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome;Department of Fundamental and Basic Sciences for Engineering, Sapienza University of Rome

关键词: satellite cells;    proliferation;    quiescence;    growth factors;    cell adhesion;    myofibers;    cell culture;   

DOI  :  10.4081/ejtm.2018.7567

学科分类：农业科学（综合）

来源: Pagepress

【 摘 要 】

Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved. This technique is an indispensable tool by which the muscle regeneration process can be recapitulated and studied in each specific phase, from satellite cell activation to proliferation, from differentiation to fusion. This study aims to clarify the effect of different culture conditions on single myofibers, their associated satellite cells, and the physiological behavior of the satellite cells upon long term culture. By direct observations of the cultures, we compared different experimental conditions and their effect on both satellite cell behavior and myofiber viability.

【 授权许可】

CC BY