Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis

Revista de microbiologia

Fonseca Júnior, Antônio Augusto¹  Sales, Mariana L.²  Orzil, Lívia³ 

[1] EMBRAPA Gado de Leite, Juiz de Fora, Brazil;Laboratório Nacional Agropecuário de Minas Gerais, Pedro Leopoldo, Brazil;Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

关键词: Mycobacterium tuberculosis;    diagnosis;    real time PCR.;   

DOI  :  10.1590/S1517-83822014000400029

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

Mycobacterium tuberculosis is the major cause of tuberculosis in humans. This bacillus gained prominence with the occurrence of HIV, presenting itself as an important opportunistic infection associated with acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification of M. tuberculosis isolates. The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0 (p < 0.001, CI = 0.84 - 1.0). The diagnostic sensitivity and specificity were 100% (CI = 95.94% - 100%) and 100% (CI = 93.98% - 100%). This qPCR was developed with the goal of diagnosing the bacillus M. tuberculosis in samples of bacterial suspension. TB reference laboratories (health and agriculture sectors), public health programs and epidemiological studies probably may benefit from such method.

【 授权许可】

CC BY-NC